Microbiology lab report on Escherichia coli and Staphylococcus aureus.

Microbiology Lab ReportPractica #1BTC307LAmber AmelingmeierThursday, September 18, 2008OBJECTIVESIn this lab experiment deuce arctic types of bacteria, Escherichia coli and Staphylococcus aureus, were grown singly and mixed on quatern assorted types of nutrient nutrient agar in order to go along the vary morphologies deep down the colonies. Resulting data was analyzed to leave alone understanding of the using up of differing culture media and conditions for bacterial ontogenesis. RESULTSFour different agar types were use in this experiment. The first ( wholesome) resigned for proceeds of some(prenominal) E. coli and S. aureus. The second agar used (MKL) inhibited the appendage of S. aureus provided allowed the growth of E. coli. The third (ash tree) pay uped growth of both bacterial species. The one-quarter (mannitol) was found to inhibit growth of E. coli (Table 1). Table 1. extension of growth within the four types of agar. G = growth, NG = no growth. E. co liS. aureusMixed cultureNutrientGGGMKLGNGGASHGGGMannitolNGGGChanges in colour within the sampled cultures were similarly noted. No deviates occurred within the Nutrient agar. The MKL agar exhibited a color change with E. coli and the mixed culture. The agar changed from red to purple, and a evident border was present contact the colonies that exhibited growth on the dish. The ASH agar did not yield a color change. The Mannitol agar exhibited a change with S. aureus and the mixed culture, but no change with E. coli (Table 2). Table 2.

Indication of color change within the four types of agar. E. coliS. aureus Mixed cultureNutrientTransparentTransparentT! ransparentMKLRed to PurpleRedRed to PurpleASHRedRedRedMannitolRedRed to YellowRed to YellowCONCLUSIONS AND DISCUSSIONTwo noticeable changes occurred during this experiment. First, two of the twelve inoculated Petri dishes yielded no growth. In order to explain these occurrences, we essential first examine the differing properties of the types of agar used. Nutrient agar is enriched to allow growth of a coarse variety... If you want to get a full essay, order it on our website: OrderCustomPaper.com

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